Generator

Part:BBa_J119305

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2013-06-13)

T5 promoter + Theo riboswitch + RFP

The combination of Riboswitch (J100065) and RFP(E1010). It was assembled using Biobrick Cloning into a pSB1A2 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal NotI site found at 7
    Illegal NotI site found at 201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal AgeI site found at 122
    Illegal AgeI site found at 774
    Illegal AgeI site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 187


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