DNA

Part:BBa_J119142

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2013-05-08)

Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI

This part is a modification of the J100091 construct that removes the C-Dog part and inserts a P4 promoter (J119030) and a C-Dog placeholder fragment (with nested BsgI sites) followed by the mRFP gene. This part is the precursor to introducing modified C-Dog parts with nested BsgI or BpmI sites to be used in Golden Gate Assembly. This part is a screening-based part and will turn red if correctly replaced by a functional C-Dog part.

This part was designed to complete the library of functional parts being used in the design of a generalized method for optimizing a metabolic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 660
    Illegal AgeI site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 48
    Illegal BsaI.rc site found at 6


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Categories
Parameters
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