Regulatory
Part:BBa_J119129
Designed by: MWSU Genetics Lab Fall 2012 Tues 8:00 Group: Eckdahl Lab (2013-01-07)
pTac with mutations
This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. A mutation was created by removing the ATTA sequence after the -35 location. This had the effect of reducing the spacing between the -35 and -10 elements from 16 base pairs to 12 base pairs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |