Composite

Part:BBa_J119125

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2012-09-28)


Ptrp+(rbs-mRFP) (J119123 ligated to S04419)

This is a part comprised of the tryptophan promoter (J119123) ligated to rbs-mRFP (S04419). It is currently cloned on a Psb1A2 plasmid in JM109 cells. It is planned to be used in the optimization of the tryptophan operon into a constitutive system uninhibited by tryptophan feedback.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
    Illegal AgeI site found at 630
    Illegal AgeI site found at 742
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None