Measurement

Part:BBa_J119108

Designed by: Michel Conn   Group: Eckdahl Lab   (2012-04-24)


pT7-RBS-GFP-pLac+tRNA CCAAU+pLac+tRNA AGGAC+pLac+tRNA CUACC

Promoter for T7 RNAP polymerase drives GFP expression. Three frameshift suppression tRNAs produced: CCAAU+AGGAC+CUACC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 989
    Illegal BamHI site found at 1348
    Illegal BamHI site found at 1707
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 1385
    Illegal AgeI site found at 1744
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


[edit]
Categories
Parameters
None