Measurement

Part:BBa_J119093

Designed by: Catherine Doyle   Group: Eckdahl Lab   (2012-04-24)


pT7-RBS-RFP-pLac+tRNA CCAAU+pLac+tRNA CUAGC+pLac+tRNA CUACC

Promoter for T7 RNAP polymerase drives RFP expression. Three frameshift suppression tRNAs produced: CCAAU+CUAGC+CUACC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 972
    Illegal BamHI site found at 1331
    Illegal BamHI site found at 1690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
    Illegal AgeI site found at 1009
    Illegal AgeI site found at 1368
    Illegal AgeI site found at 1727
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None