Reporter

Part:BBa_J119089

Designed by: Hannah Huff, Andie Cassity   Group: Eckdahl Lab   (2012-03-28)


UV promoter w/ RBS and lacZ gene

This part consists of a UV promoter attached to the lacZ gene with a RBS in front of it. The promoter is activated by ultra-violet light to transcribe the lacZ gene. When plated with X-gal, the part should function as a reporter for exposure to UV light by the mechanism of lacZ cleaving X-gal to turn blue.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None