Project

Part:BBa_J119077

Designed by: Michel Conn   Group: Eckdahl_Lab   (2012-03-08)


tRNA-pLacI-RBS-T7RNAP (mutant 4 and tRNA 91)

This part contains T7RNAP with an inserted oligo causing a frameshift mutation. The tRNA is a five base frameshift suppressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 209
    Illegal BamHI site found at 568
    Illegal BamHI site found at 927
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 246
    Illegal AgeI site found at 605
    Illegal AgeI site found at 964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1225
    Illegal BsaI.rc site found at 1176


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Categories
Parameters
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