Reporter

Part:BBa_J119075

Designed by: Jerrad Morton   Group: Eckdahl_Lab   (2012-02-11)


pT7-RBS-RFP-pLacI-RBS-T7RNAP

T7 RNAP produced from pLacI promoter. T7 RNAP drives expression of RFP from the pT7 promoter. T7 RNAP gene has sites for insertion of frameshift mutations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 889
    Illegal BsaI.rc site found at 862


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