Reporter

Part:BBa_J119068

Designed by: Eric Sawyer   Group: Eckdahl Lab   (2012-02-02)

RFPrev-RBSrev-pT7rev-pLacI-RBS-T7 RNAP (2SAT mutant 1)

RFP expression driven by pT7 promoter. T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (a’ or b) and (a or c’).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
    Illegal AgeI site found at 147
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 911
    Illegal BsaI.rc site found at 862


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