Plasmid_Backbone

Part:BBa_J119061

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2012-01-27)


pSB1A2-BR

Plasmid pSB1A2 with BsaI site removed from Ampicillin resistance gene. For use in Golden Gate Assembly with BsaI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2058
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2058
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2073
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None