Coding

Part:BBa_J119056

Designed by: Michel Conn   Group: Eckdahl Lab   (2012-01-26)

pLacI-RBS-T7 RNAP (2SAT mutant 2)

T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (b’ or c’)and(a or b).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 148
    Illegal BsaI.rc site found at 99


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