RBS
Part:BBa_J119053
Designed by: David Carr Group: Eckdahl_Lab (2012-01-25)
J23100 Promoter with BD18 RBS w/ leader and RFP reporter
The sequence contains a J23100 promoter followed by the BD18 leader RBS. The promoter has two BsaI sites, one at each end of the sequence. The one on the 5' end of J23100 cuts to the left, and the one at the 3' end of J23100 cuts to the right. There are also two BbsI sites, one is up stream from the 5' BsaI site and cuts to the left, while the other is down stream from the 3' BsaI site and cuts to the right. This is followed by an RFP gene starting with the second codon.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 850 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 39
Illegal NheI site found at 62
Illegal SpeI site found at 851
Illegal PstI site found at 865
Illegal NotI site found at 7
Illegal NotI site found at 858 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 851 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 851
Illegal PstI site found at 865
Illegal AgeI site found at 723
Illegal AgeI site found at 835 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |