Regulatory

Part:BBa_J119052

Designed by: David Carr   Group: Eckdahl_Lab   (2012-01-25)


J23100 promoter for BsaI GGA

J23100 promoter flanked by BsaI sites for use in Golden Gate Assembly. The 5' word is CGAC and the 3' word is GCGG. The part can be assembled into J119023 using these words.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
    Illegal NheI site found at 41
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 52


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