Plasmid_Backbone

Part:BBa_J119051

Designed by: Todd Eckdahl and A. Malcolm Campbell   Group: Eckdahl Lab   (2012-01-23)


pSB4T8

Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3935
    Illegal NheI site found at 2743
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3941
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3935
    Illegal BamHI site found at 2889
    Illegal XhoI site found at 2508
    Illegal XhoI site found at 3791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3935
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3935
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3950
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2915
    Illegal NgoMIV site found at 3283
    Illegal NgoMIV site found at 3443
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None