Plasmid_Backbone
Part:BBa_J119048
Designed by: A. Malcolm Campbell and Todd Eckdahl Group: Eckdahl Lab (2012-01-23)
pSB4A8
Insulated low copy vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix. Uses I50042 low copy pSC101 replication origin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 3629
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3629
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3635 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3629 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3629
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3629
Plasmid lacks a suffix.
Illegal XbaI site found at 3644
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
[edit]
Categories
Parameters
None |