Plasmid_Backbone

Part:BBa_J119047

Designed by: A. Malcolm Campbell and Todd Eckdahl   Group: Eckdahl Lab   (2012-01-23)


pSB1T8

Insulated vector based on pSB1A2. Uses B1010 transcriptional terminator upstream of BBa prefix and B0025 double transcription terminator downstream of BBa suffix.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2513
    Illegal NheI site found at 1321
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2519
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2513
    Illegal BamHI site found at 1467
    Illegal XhoI site found at 1086
    Illegal XhoI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2513
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2513
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1493
    Illegal NgoMIV site found at 1861
    Illegal NgoMIV site found at 2021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None