Composite

Part:BBa_J119038

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2012-01-02)


START(pTet)-GFP1

Half-edge for use in assembly of Hamiltonian Path Problem graphs. Composed of START half-edge and GFP1 half-edge. Contains START word and GFP gene-specific word that become available for ligation upon digestion with BsaI during Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 460
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 460
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 460
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 454


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Categories
Parameters
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