Reporter

Part:BBa_J119016

Designed by: Michel Conn   Group: Eckdahl Lab   (2011-10-07)


pLac-RBS-T7RNAP-pT7-RBS-RFP

This gene was created to be a self promoter. The T7RNAP gene product is T7RNAP which binds to the pT7 promoter and cause the cell to fluoresce red.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3417
    Illegal AgeI site found at 3529
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 126
    Illegal BsaI.rc site found at 99


[edit]
Categories
Parameters
None