DNA

Part:BBa_J119014

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2011-08-18)


HPP assembly vector

New HPP vector for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. EcoRI site followed by the START sticky word made available by the following BsaI site pointing left. Followed by NheI, then another BsaI site pointing right that frees up the FINISH sticky word. Followed by the PstI site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 17
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 11


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Categories
Parameters
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