DNA

Part:BBa_J119013

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2011-08-18)


FINISH

Finish half edge for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. BsmBI site pointing right that when digested frees up half edge word "GTGG" on the 5' end that allows ligation to any gene 2 half-edge. Followed by the BsaI site pointing left that when digested frees up the FINISH word "CGAG" that allows ligation to the new tr5f4HPP vector. In between these two sites is an 8 nt spacer. This section is flanked by EcoRI and PstI sticky ends for cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 30


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