Reporter

Part:BBa_J119012

Designed by: Michel Conn   Group: Eckdahl Lab   (2011-08-16)


pT7-RBS-LacZ-pLac-RBS-T7RNAP

This part consists of both T7RNAP and the T7RNAP promoter. The gene produces a blue color when on a plate that contains X-gal. The gene produces T7RNAP which attaches to the T7RNAP promoter this produces LacZ and a blue color.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3258
    Illegal BsaI.rc site found at 3231


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Categories
Parameters
None