DNA
Part:BBa_J119011
Designed by: Zachary Caton Group: Eckdahl Lab (2011-08-03)
BsaI-GFP2 (BsaI removed)-BsmBI
This is GFP-2A, the second half of the GFP Gene Template to be used in conjunction with GFP-1 to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a green fluorescence. Can be used with other split genes to solve more complex HPP problems.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
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Categories
Parameters
None |