DNA

Part:BBa_J119011

Designed by: Zachary Caton   Group: Eckdahl Lab   (2011-08-03)

BsaI-GFP2 (BsaI removed)-BsmBI

This is GFP-2A, the second half of the GFP Gene Template to be used in conjunction with GFP-1 to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a green fluorescence. Can be used with other split genes to solve more complex HPP problems.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7


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Categories
Parameters
None