DNA

Part:BBa_J119007

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2011-07-22)


HPP Assembly Vector pSB1A2 with Promoter-BsmBI-BsaI

Vector for use in Golden Gate Assembly of Hamiltonian Path Problem constructs. EcoRI site on the left. Then there is a J23100 promoter that points to the right. It is followed by a half-edge sticky word of GTGG, made available by a BsmBI site pointing to the left. Next is a BsaI site pointing to the right that frees up the following half-edge sticky word of GTGG. Finally, there is a PstI site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 68
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 13
    Illegal NheI site found at 36
    Illegal PstI site found at 68
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 68
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 68
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 57


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Categories
Parameters
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