Part:BBa_J119004

Second half of the LacZ Gene (LacZ-2), split for use in HPP (Hamiltonian Path Problems). It is the necessary complement half of BBa_J119003, without the other, neither will function. They are intended to be re-assembled via transformation in E Coli JM109. It functions as a reporter gene when properly assembled with LacZ-1 by producing blue coloring (non-fluorescing) when X-Gal is present. Can be used in conjunction with other split genes but must have the complement to report properly.

Beta-Gal_2_For (36mer)

5’ GCAT GAATTC GGTCTC A CCTG GAGAGGCGCGCCCGC 3’

(4, EcoRI , BsaI , a , Gene1234 , 15mer of Beta-Gal)

Beta-Gal_2_Rev (96mer)

5’ATGC CTGCAG CGTCTC A CCAC CCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACG TTGC TTATTTTTGACACCA 3’

(4 , PstI , BsmBI , a , Halfedge1234RC , pLacI rc , 4mer ,

15mer of Beta-Gal Rev)