Part:BBa_J119003

First half of the LacZ Gene (LacZ-1), split for use in HPP (Hamiltonian Path Problems). It is the necessary complement half of BBa_J119004, without the other, neither will function. They are intended to be re-assembled via transformation in E Coli Jm109. It functions as a reporter gene when properly assembled with LacZ-2 by producing blue coloring (non-fluorescing) when X-Gal is present. Can be used in conjunction with other split genes but must have the complement to report properly.

Beta-Gal_1_For (58mer) 5’ GCAT GAATTC CGTCTC A GTGG CA AAAGAGGAGAAA CGTACGAC ATGACCATGATTACG 3’

(4nt , EcoRI, BsmBI , a , Halfedge1234, nn, RBS 0034 , 8 , 15mer of Beta-Gal)

Beta-Gal_1_Rev (36mer)

5’ATGC CTGCAG GGTCTC A CAGG TAGCGAAAGCCATTT 3’)

(4nt, PstI , BsaI , a , Gene1234RC , 15mer of Beta-Gal reverse)