Intermediate
B_whiteboa

Part:BBa_J11030

Designed by: Seema Nagaraj   Group: iGEM2005   (2005-10-03)


Bacterial Whiteboard by Hannah

When lactose is added to the system, bind to lac repressor (endogenous) therefore GFP produced - glow green. When Tet added to the system, drive transcription of lacZ beta galactosidase which hydrolyses latose and lac repressor active. Stop Glowing green.

      • NOTE*** This system will ONLY WORK IN LAC Z- bugs!!!!!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 173
    Illegal XhoI site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1306


[edit]
Categories
//classic/intermediate/uncategorized
Parameters
None