Project
TTSv3

Part:BBa_J11022

Designed by: Emanuel Nazareth   Group: iGEM2005   (2005-10-05)


Tunable Temperature Sensor v3 (Registry 7.05 DNA-1 + Harvard Parts)

Tuneable Temperature Sensor made with parts available in Registry 7.05 DNA-1. At higher temperatures, the lacI dimer degrades into lacI monomers. The LacI promoter is no longer inhibited, hence more RFP is produced. Also, cI 434 is produced resulting in Prm+ being repressed, thus lowering the levels of GFP. At lower temperatures, the lacI dimer is allowed to form, hence the LacI promoter is repressed. RFP levels decrease, and cI levels drop. This results in Prm+ no longer being repressed, hence GFP levels increase. Behaviour is tuneable by adjusting the levels of lacI ts via L-arabinose activating pBad. The net result is that the fluorescence (green to red) can be correlated to a temperature (at a given level of L-arabinose).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4179
    Illegal AgeI site found at 4291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5228
    Illegal SapI site found at 961


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