Temporary

Part:BBa_J11008

Designed by: Andrew Hessel   Group: iGEM2005   (2005-07-27)


variant of J11004 (hi temp color) with weaker RBS

Variant of BBa_J1104 with weaker RBS. Concern is that GFP signal will be too strong. The expression of GFP should not start until 37C, but a weaker promoter will help ensure this.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 721


[edit]
Categories
//classic/temporary/uncategorized
Parameters
None