DNA
pSC101ts

Part:BBa_J107112

Designed by: Lorenzo Pasotti   Group: Magni Lab   (2016-01-02)


pSC101ts origin with SpeI removed

Temperature-sensitive pSC101 replication origin without SpeI site. This part was used to design the pBBknock BioBrick vector for allelic replacement (BBa_J107077).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1438
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None