Plasmid_Backbone
pBBknock

Part:BBa_J107077

Designed by: Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolo Politi, Ilaria Massaiu, Cinzia Calvio, Gabriella Cusella, Paolo Magni   Group: Magni Lab   (2016-01-02)


pBBknock standard vector for allelic replacement

This vector can be specialized to target the desired locus in the Escherichia coli genome and carry out allelic replacement, e.g., knockout, chromosomal integration, mutagenesis, etc. See Casanova et al. (2016), Biol Proced Online for detailed instructions and protocols.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3865
    Illegal NheI site found at 28
    Illegal NheI site found at 51
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2521
    Illegal NotI site found at 3871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3865
    Illegal BglII site found at 1023
    Illegal XhoI site found at 3090
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3865
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3865
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3880
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 573
    Illegal AgeI site found at 924
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1070


[edit]
Categories
Parameters
None