Plasmid_Backbone

Part:BBa_J107058

Designed by: Lorenzo Pasotti   Group: Magni Lab   (2012-08-26)


BioBrick integrative vector targeting aspA locus

It has been derived from BBa_K300000 base vector by exciding the default integration guide (attP of phi80 phage) and ligating aspA (BBa_C0083) as guide.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 169
    Illegal XbaI site found at 1539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3258
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3258
    Illegal BglII site found at 3006
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3258
    Illegal suffix found at 2
    Illegal XbaI site found at 169
    Illegal XbaI site found at 1539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3258
    Plasmid lacks a suffix.
    Illegal XbaI site found at 169
    Illegal XbaI site found at 1539
    Illegal XbaI site found at 3273
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2114
    Illegal AgeI site found at 1733
    Illegal AgeI site found at 2585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2073


[edit]
Categories
Parameters
None