Measurement

Part:BBa_J107038

Designed by: Lorenzo Pasotti   Group: Magni_UNIPV_Lab   (2013-07-15)


HSL-inducible device + NOT gate + RFP

LuxR is expressed by the PLlacO1 promoter. In presence of HSL, LuxR activates Plux, which produces TetR. In this case PtetR is inhibited. In absence of HSL, PtetR is ON.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2555
    Illegal AgeI site found at 2667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


[edit]
Categories
Parameters
None