Reporter

Part:BBa_J10068

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


luciferase + LRE + BD18 original RBS removed

Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)

The original part (K325229) was used as the template. We have removed all aspects of the pBad promoter and AraC regulatory DNA as well as the original first RBS. This part now starts with BD18 (J119024).

K325229 is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Val239Ile mutation was used to create a green bioluminescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2621
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 2057
    Illegal SpeI site found at 2622
    Illegal PstI site found at 2636
    Illegal NotI site found at 2629
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2622
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2622
    Illegal PstI site found at 2636
    Illegal AgeI site found at 897
    Illegal AgeI site found at 2599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal SapI.rc site found at 329
    Illegal SapI.rc site found at 2113


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