Part:BBa_J10064

Lux Operon Vibrio fischeri from part K325909

This page described the lux operon from Vibrio fischeri. To relieve LuxR control we placed Lux C, D, A, B, E under a promoter of your choice. A more complete description can be found on the [[http://2010.igem.org/Team:Cambridge Cambridge iGEM 2010] website.

This Bacterial lux operons encodes five enzymes involved in the light-generating pathway. LuxA and LuxB encode the two subunits of the bacterial luciferase, while the products of LuxC, LuxD and LuxE synthesise the substrate for the light emitting reaction, tetradecanal. The exact function of LuxG is unknown, and it appears to be non-essential for light emission, but its presence increases light output.

In nature, the lux genes appear to be repressed by the nucleoid protein, H-NS, and occur under quorum sensing control. We removed the natural quorum sensing control to facilitate use of the part in biosensors under different regulatory inputs.

As of October 2010 [http://2010.igem.org/Team:Cambridge Cambridge] believe this is the first and only BioBrick to emit light in normal E. coli strains without the addition of any external substrate.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2961
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1959
  Illegal XhoI site found at 2789
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4348
  Illegal BsaI.rc site found at 1357
  Illegal SapI.rc site found at 4673