Reporter

Part:BBa_J100314

Designed by: Monica Prudencio   Group: Campbell M Lab   (2017-01-19)


actClone Red (J100204) with scrambled OmpR promoter (J100313)

We inserted a non-functional version of the OmpR promoter in actClone Red. Because the OmpC binding sites have been scrambled, the promoter does not function to allow transcription of the downstream RFP. We will use this part as a negative control when using actClone Red to test the effects of modifications to the OmpR promoter on its ability to promote transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 841
    Illegal SpeI site found at 868
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 702


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