Reporter

Part:BBa_J100312

Designed by: Monica Prudencio   Group: Campbell M Lab   (2016-12-02)


repClone Red (J100205) with scrambled TetR promoter (J100311)

We inserted a non-functional version of the TetR repressible promoter in repClone Red. Because the TetR binding sites have been scrambled, the promoter does not function to allow transcription of the downstream RFP, regardless of the presence of aTc. We will use this part as a negative control when using repClone Red to test the effects of modifications to the TetR promoter on its ability to promote transcription with or without aTc present.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2212
    Illegal AgeI site found at 2324
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None