Regulatory

Part:BBa_J100299

Designed by: Lydia Soifer   Group: Campbell M Lab   (2016-09-08)


lysine regulated promoter

This promoter includes the -91 to -81 regions directly upstream of the -35 to 0 region in addition to the sticky ends for BsaI. Top1- 5' CGACTTTCACTGATATGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACG Bot1- 5' CGCCCGTTTTCAGAGTAACCAGCGCATCGCTACGCCGCCATATCAGTGAAA The -81 to -118 region is known to be a binding sight for lysine repression of the promoter (Bouvier, Jean, et. al 2008). If this whole region is not needed for lysine binding and the lysine will still bind if the binding sight is located directly upstream of the -35 region, the promoter should be activated in the absence of lysine and repressed in the presence of lysine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None