Regulatory
Part:BBa_J100299
Designed by: Lydia Soifer Group: Campbell M Lab (2016-09-08)
lysine regulated promoter
This promoter includes the -91 to -81 regions directly upstream of the -35 to 0 region in addition to the sticky ends for BsaI. Top1- 5' CGACTTTCACTGATATGGCGGCGTAGCGATGCGCTGGTTACTCTGAAAACG Bot1- 5' CGCCCGTTTTCAGAGTAACCAGCGCATCGCTACGCCGCCATATCAGTGAAA The -81 to -118 region is known to be a binding sight for lysine repression of the promoter (Bouvier, Jean, et. al 2008). If this whole region is not needed for lysine binding and the lysine will still bind if the binding sight is located directly upstream of the -35 region, the promoter should be activated in the absence of lysine and repressed in the presence of lysine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |