Plasmid

Part:BBa_J100288

Designed by: Hartlee Johnston   Group: Campbell M Lab   (2016-07-11)


pJC173b with gIII neg

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 (pJC173b) to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat. This construct is based on J100265 and contains a deletion of 210 bp, encoding 70 amino acids, from gene III. The mutant gene III encodes a protein III that acts as a dominant negative for M13 phage reproduction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
    Illegal AgeI site found at 3407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5247
    Illegal SapI site found at 2648
    Illegal SapI site found at 3926


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Categories
Parameters
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