Plasmid

Part:BBa_J100287

Designed by: Owen Koucky   Group: Campbell_M_Lab   (2016-07-10)


J100265 (pJC173b) with GFP replacing LuxAB

In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat. In order to conduct a PACE experiment of our own we used PCR and GGA to replace the LuxAB gene on part J100265 with the GFP gene to construct part J100287.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1812
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4050


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