Plasmid

Part:BBa_J100285

Designed by: Dylan Maghini   Group: Campbell M Lab   (2016-07-08)


SPT7specific with Riboswitch C

This is the SPT7specific plasmid with translational riboswitch C inserted. It will be used in the positive and negative phases of the Campbell lab's phage assisted continuous evolution experiment. In the experiment, we will apply pressure on the theophylline riboswitch to evolve into a xanthine specific riboswitch. This is a translational riboswitch.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5521
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5521
    Illegal NgoMIV site found at 8483
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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