Plasmid

Part:BBa_J100284

Designed by: Zachary Shaver   Group: Campbell M Lab   (2016-07-06)


JC184d5 with Mutagenesis Cassette Removed

This part is the plasmid JC184d5 with the mutagenesis cassette removed. It is used in the negative selection phase of PACE to allow for survival of the desired phage with the addition of aTc. The mutagenesis cassette is removed to prevent a high rate of mutagenesis in the negative selection phase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2962
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1525
    Illegal NotI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1407
    Illegal BamHI site found at 2194
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2962
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2962
    Illegal PstI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1059


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Categories
Parameters
None