Measurement

Part:BBa_J100280

Designed by: Anna Buser   Group: Campbell M Lab   (2016-06-24)


tClone Tet+PDB riboswitch with P9 and BD2

The BD2 C Dog Ribosome Binding Site has a lower binding efficiency than BD18 and BD10. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100275 and insert in the BD2 C Dog RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 496
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 522
    Illegal NgoMIV site found at 890
    Illegal NgoMIV site found at 1050
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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