Measurement

Part:BBa_J100273

Designed by: Anna Buser   Group: Campbell M Lab   (2016-06-24)


tCloneTet+PDB riboswitch with P2

The tCloneTet+PDB plasmid was obtained from part J100255 and was modified using Golden Gate Assembly to have a P2 promoter rather than the P5 promoter. The P2 promoter is known to have a lower efficiency than P5 but it is still relatively high compared to other promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 901
    Illegal NgoMIV site found at 1061
  • 1000
    COMPATIBLE WITH RFC[1000]


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