Plasmid

Part:BBa_J100265

Designed by: Owen Koucky   Group: Campbell M Lab   (2016-06-17)


pJC173b (Carlson et al. 2014)

In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100265 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2482
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1018
    Illegal PstI site found at 1813
    Illegal AgeI site found at 3618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5458
    Illegal SapI site found at 4137


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Categories
Parameters
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