Reporter

Part:BBa_J100263

Designed by: Owen Koucky   Group: Campbell_M_Lab   (2016-06-09)


RS10 (Wachsmuth et al. 2013) in J119361

J11263 reports the functionality of the RS10 riboswitch from Wachsumth et al. 2013 by its expression of the reporter RFP gene. The RS10 riboswitch is a theophylline-binding transcriptional riboswitch that turns on gene expression in the presence of theophylline. In the presence of theophylline, cells containing this part should fluoresce red. In the absence of theophylline, cells containing this part should not fluoresce.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 752
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None