Reporter

Part:BBa_J100262

Designed by: Zachary Shaver   Group: Campbell M Lab   (2016-06-08)


rClone Red with Riboswitch E

This part is a modified form of the rClone Red plasmid (part BBa_J119384) that replaced the GFP section flanked by BsaI sites with a theophylline riboswitch, labeled riboswitch E, by Golden Gate Assembly (GGA). In the presence of theophylline, the cells should fluoresce red. In the absence of theophylline, the cells should not fluoresce.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 655
    Illegal AgeI site found at 767
  • 1000
    COMPATIBLE WITH RFC[1000]


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