Measurement

Part:BBa_J100259

Designed by: Shuk Hang Li   Group: Campbell M Lab   (2016-06-08)


DL Nitrate Biosensor

This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by trimming the left Fis binding site, where an inhibitory regulator bind. The modified promoter is inserted into pClone Red (J119137). The goal is that nitrate induces the promoter, then RFP is expressed when nitrate is present. However, this promoter does not induce RFP production when nitrate is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1515
    Illegal AgeI site found at 1627
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None