Measurement
Part:BBa_J100259
Designed by: Shuk Hang Li Group: Campbell M Lab (2016-06-08)
DL Nitrate Biosensor
This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by trimming the left Fis binding site, where an inhibitory regulator bind. The modified promoter is inserted into pClone Red (J119137). The goal is that nitrate induces the promoter, then RFP is expressed when nitrate is present. However, this promoter does not induce RFP production when nitrate is present.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1515
Illegal AgeI site found at 1627 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |