Measurement

Part:BBa_J100256

Designed by: Shuk Hang Li   Group: Campbell M Lab   (2016-05-16)


L Nitrate Biosensor (left)

This construct is built by modifying the promoter sequence of Original Nitrate Biosensor (J100237), which inserts narG promoter sequence into pClone Red (J119137) through GGA. The promoter sequence is modified by disrupting the left Fis binding site, where an inhibitory regulator binds; moreover,since there is an overlap between the activator and inhibitor binding site, we had to make sure the activator can still bind on the same site. The modified promoter is inserted in to pClone Red (J119137). Nitrate induces the promoter, so RFP is expressed when nitrate is present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1673
    Illegal AgeI site found at 1785
  • 1000
    COMPATIBLE WITH RFC[1000]


bbb

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