Device

Part:BBa_J100252

Designed by: Helen Webster   Group: Campbell M Lab   (2016-03-23)

tCloneTet with RS10shift

Started with tCloneRed (J119361) and used Golden Gate Assembly to remove GFP reporter and put transcriptional terminator riboswitch RS10shift (RS10s). RS10s was taken from the Wachsmuth et. al. 2013 NAR vol. 41 p. 2541. This riboswitch works and is responsive to theophylline (2.5mM).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 773
    Illegal AgeI site found at 885
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None